Faculty Profile

Calvin Henard

Title
Assistant Professor
Department
Biological Sciences
College
College of Science

    

    

      


                          

            
Curriculum Vitae

            

              Click Here to View Faculty CV
            

          

        
                          

            
Education

            



  

  


  
PhD, University of Colorado, 2012. 
Major: Microbiology
Dissertation Title: Mediated Resistance of Salmonella Against Oxidative and Nitrosative Stress
BS, Tarleton State University, 2006. 
Major: Molecular Biology





          

        
                

          
Current Scheduled Teaching*


          
BIOC 6620.001, Advanced Cell Biology, Spring 2024 
BIOL 6620.001, Advanced Cell Biology, Spring 2024 
BIOC 6940.716, Individual Research, Spring 2024 
BIOC 6900.716, Special Problems, Spring 2024 
BIOL 4900.716, Special Problems, Spring 2024 


          
*
  Texas Education Code 51.974 (HB 2504) requires each institution of
  higher education to make available to the public, a syllabus for
  undergraduate lecture courses offered for credit by the institution.
  

        

      

    

  


    

    

      


        


          


            
Previous Scheduled Teaching*


            
                    

                    BIOL 4501.001, Bacterial Diversity and Physiology, Fall 2023 Syllabus SPOT
                    

                    BIOL 5501.001, Bacterial Diversity and Physiology, Fall 2023 Syllabus SPOT
                    

                    BIOL 4502.501, Bacterial Diversity and Physiology Laboratory, Fall 2023 Syllabus SPOT
                    

                    BIOL 4502.502, Bacterial Diversity and Physiology Laboratory, Fall 2023 Syllabus SPOT
                    

                    BIOL 5502.502, Bacterial Diversity and Physiology Laboratory, Fall 2023 Syllabus SPOT
                    

                    BIOC 4570.001, Biochemistry and Molecular Biology of the Gene, Fall 2023 Syllabus SPOT
                    

                    BIOL 4570.001, Biochemistry and Molecular Biology of the Gene, Fall 2023 Syllabus SPOT
                    

                    BIOC 6950.716, Doctoral Dissertation, Fall 2023 
                    

                    BIOC 6940.716, Individual Research, Fall 2023 
                    

                    BIOL 4900.716, Special Problems, Fall 2023 
                    

                    BIOL 6900.716, Special Problems, Fall 2023 
                    

                    BIOC 4570.001, Biochemistry and Molecular Biology of the Gene, Spring 2023 Syllabus SPOT
                    

                    BIOC 5340.001, Biochemistry and Molecular Biology of the Gene, Spring 2023  SPOT
                    

                    BIOL 4570.001, Biochemistry and Molecular Biology of the Gene, Spring 2023 Syllabus SPOT
                    

                    BIOL 5340.001, Biochemistry and Molecular Biology of the Gene, Spring 2023  SPOT
                    

                    BIOC 6940.716, Individual Research, Spring 2023 
                    

                    BIOL 4501.001, Bacterial Diversity and Physiology, Fall 2022 Syllabus SPOT
                    

                    BIOL 5501.001, Bacterial Diversity and Physiology, Fall 2022  SPOT
                    

                    BIOL 4502.501, Bacterial Diversity and Physiology Laboratory, Fall 2022 Syllabus SPOT
                    

                    BIOL 4502.502, Bacterial Diversity and Physiology Laboratory, Fall 2022 Syllabus SPOT
                    

                    BIOC 6940.716, Individual Research, Fall 2022 
                    

                    BIOC 4570.001, Biochemistry and Molecular Biology of the Gene, Spring 2022 Syllabus SPOT
                    

                    BIOC 5340.001, Biochemistry and Molecular Biology of the Gene, Spring 2022  SPOT
                    

                    BIOL 4570.001, Biochemistry and Molecular Biology of the Gene, Spring 2022 Syllabus SPOT
                    

                    BIOL 5340.001, Biochemistry and Molecular Biology of the Gene, Spring 2022  SPOT
                    

                    BIOC 6940.716, Individual Research, Spring 2022 
                    

                    BIOL 4900.716, Special Problems, Spring 2022 
                    

                    BIOL 4501.001, Bacterial Diversity and Physiology, Fall 2021 Syllabus SPOT
                    

                    BIOL 5501.001, Bacterial Diversity and Physiology, Fall 2021  SPOT
                    

                    BIOL 4502.501, Bacterial Diversity and Physiology Laboratory, Fall 2021 Syllabus SPOT
                    

                    BIOL 4502.502, Bacterial Diversity and Physiology Laboratory, Fall 2021 Syllabus SPOT
                    

                    BIOL 5502.501, Bacterial Diversity and Physiology Laboratory, Fall 2021  SPOT
                    

                    BIOL 6940.716, Individual Research, Fall 2021 
                    

                    BIOL 4900.716, Special Problems, Summer 10W 2021 
                    

                    BIOC 4570.001, Biochemistry and Molecular Biology of the Gene, Spring 2021 Syllabus SPOT
                    

                    BIOC 5340.001, Biochemistry and Molecular Biology of the Gene, Spring 2021  SPOT
                    

                    BIOL 4570.001, Biochemistry and Molecular Biology of the Gene, Spring 2021 Syllabus SPOT
                    

                    BIOL 5340.001, Biochemistry and Molecular Biology of the Gene, Spring 2021  SPOT
                    

                    BIOC 6940.716, Individual Research, Spring 2021 
                    

                    BIOC 5900.716, Special Problems, Spring 2021 
                    

                    BIOC 5910.716, Special Problems, Spring 2021 
                    

                    BIOL 4900.716, Special Problems, Spring 2021 
                    

                    BIOL 4910.716, Special Problems, Spring 2021 
                    

                    BIOL 4501.001, Bacterial Diversity and Physiology, Fall 2020 Syllabus SPOT
                    

                    BIOL 5501.001, Bacterial Diversity and Physiology, Fall 2020  SPOT
                    

                    BIOL 4502.501, Bacterial Diversity and Physiology Laboratory, Fall 2020 Syllabus SPOT
                    

                    BIOC 6940.716, Individual Research, Fall 2020 
                    

                    BIOL 6940.716, Individual Research, Fall 2020 
                    

                    BIOC 4900.716, Special Problems, Fall 2020 
                    

                    BIOL 4900.716, Special Problems, Fall 2020 
                    

                    BIOC 4570.001, Biochemistry and Molecular Biology of the Gene, Spring 2020 Syllabus
                    

                    BIOC 5340.001, Biochemistry and Molecular Biology of the Gene, Spring 2020 Syllabus
                    

                    BIOL 4570.001, Biochemistry and Molecular Biology of the Gene, Spring 2020 Syllabus
                    

                    BIOL 5340.001, Biochemistry and Molecular Biology of the Gene, Spring 2020 Syllabus
                    

                    BIOC 6940.716, Individual Research, Spring 2020 
                    

                    BIOC 6900.716, Special Problems, Spring 2020 
                    

                    BIOL 6940.516, Individual Research, Fall 2019 

          


          
*
  Texas Education Code 51.974 (HB 2504) requires each institution of
  higher education to make available to the public, a syllabus for
  undergraduate lecture courses offered for credit by the institution.
  

        

      

    

  

  
  	

	

	  

		

		   
Published Publications



  

  
Published Intellectual Contributions


  
Book Chapter
Nath, S., Henard, J. M., Henard, C. A. (2022). Optimized Tools and Methods for Methanotroph Genome Editing. Methods in Molecular Biology. 2489, 421-434. https://api.elsevier.com/content/abstract/scopus_id/85130002297
Knoshaug, E. P., Gerritsen, A. T., Henard, C. A., Guarnieri, M. T. (2020). Methods for algal protein isolation and proteome analysis. Methods in Molecular Biology. 2096, 51-59. https://api.elsevier.com/content/abstract/scopus_id/85088812869
Henard, C. A., Guarnieri, M. T. (2018). Metabolic engineering of methanotrophic bacteria for industrial biomanufacturing. Methane Biocatalysis: Paving the Way to Sustainability. 117-132. https://api.elsevier.com/content/abstract/scopus_id/85054123247
Commentary
Henard, C. A. (2023). Insights into methanotroph carbon flux pave the way for methane biocatalysis. Trends in Biotechnology. 41(3), . 
Editorial, Journal
Xiong, W., Kalyuzhnaya, M. G., Henard, C. A. (2021). Editorial: Microbial C1 Metabolism and Biotechnology. Frontiers in Microbiology. 12, . https://api.elsevier.com/content/abstract/scopus_id/85114750879
Journal Article
Henard, C. A., Wu, C., Xiong, W., Henard, J. M., Davidheiser-Kroll, B., Orata, F. D., Guarnieri, M. T. (2021). Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase (RubisCO) Is Essential for Growth of the Methanotroph Methylococcus capsulatus Strain Bath. Applied and Environmental Microbiology. 87(18), 1-13. https://api.elsevier.com/content/abstract/scopus_id/85116509424
Fei, Q., Liang, B., Tao, L., Tan, E. C., Gonzalez, R., Henard, C. A., Guarnieri, M. T. (2020). Biological valorization of natural gas for the production of lactic acid: Techno-economic analysis and life cycle assessment. Other. 158, . https://api.elsevier.com/content/abstract/scopus_id/85082456936
Dahlin, L. R., Gerritsen, A. T., Henard, C. A., Van Wychen, S., Linger, J. G., Kunde, Y., Hovde, B. T., Starkenburg, S. R., Posewitz, M. C., Guarnieri, M. T. (2019). Development of a high-productivity, halophilic, thermotolerant microalga Picochlorum renovo. Other. 2(1), . https://api.elsevier.com/content/abstract/scopus_id/85074150358
Henard, C., Akberdin, I., Kalyuzhnaya, M., Guarnieri, M.  (2019). Muconic acid production from methane using rationally-engineered methanotrophic biocatalysts. Green Chemistry. (21), 6731-6737. Royal Society of Chemistry. https://pubs.rsc.org/en/content/articlehtml/2019/gc/c9gc03722e
Qian, F., Zhu, C., Knipe, J. M., Ruelas, S., Stolaroff, J. K., Deotte, J. R., Duoss, E. B., Spadaccini, C. M., Henard, C. A., Guarnieri, M. T., Baker, S. E. (2019). Direct Writing of Tunable Living Inks for Bioprocess Intensification. Nano Letters. 19(9), 5829-5835. https://api.elsevier.com/content/abstract/scopus_id/85062327086
Tapscott, T., Guarnieri, M. T., Henard, C. A. (2019). Development of a CRISPR/Cas9 system for Methylococcus capsulatus in vivo gene editing. Applied and Environmental Microbiology. 85(11), . https://api.elsevier.com/content/abstract/scopus_id/85066426544
Akberdin, I. R., Thompson, M., Hamilton, R., Desai, N., Alexander, D., Henard, C. A., Guarnieri, M. T., Kalyuzhnaya, M. G. (2018). Methane utilization in Methylomicrobium alcaliphilum 20Z<sup>R</sup>: A systems approach. Scientific Reports. 8(1), . https://api.elsevier.com/content/abstract/scopus_id/85041644582
Fitzsimmons, L., Liu, L., Porwollik, S., Chakraborty, S., Desai, P., Tapscott, T., Henard, C. A., McClelland, M., Vazquez-Torres, A.  (2018). Zinc-dependent substrate-level phosphorylation powers Salmonella growth under nitrosative stress of the innate host response. Other. 14(10), . https://api.elsevier.com/content/abstract/scopus_id/85056394389
Guarnieri, M. T., Levering, J., Henard, C. A., Boore, J. L., Betenbaugh, M. J., Zengler, K., Knoshaug, E. P. (2018). Genome sequence of the oleaginous green alga, Chlorella vulgaris UTEX 395. Other. 6(APR), . https://api.elsevier.com/content/abstract/scopus_id/85046631237
Guarnieri, M. T., Gerritsen, A. T., Henard, C. A., Knoshaug, E. P. (2018). Phosphoproteome of the oleaginous green alga, Chlorella vulgaris UTEX 395, under nitrogen-replete and -deplete conditions. Other. 6(MAR), . https://api.elsevier.com/content/abstract/scopus_id/85044975794
Shaw, J. A., Henard, C. A., Liu, L., Dieckman, L. M., Vázquez-Torres, A., Bourret, T. J. (2018). Salmonella enterica serovar Typhimurium has three transketolase enzymes contributing to the pentose phosphate pathway. Journal of Biological Chemistry. 293(29), 11271-11282. https://api.elsevier.com/content/abstract/scopus_id/85050406193
Henard, C. A., Smith, H. K., Guarnieri, M. T. (2017). Phosphoketolase overexpression increases biomass and lipid yield from methane in an obligate methanotrophic biocatalyst. Other. 41, 152-158. https://api.elsevier.com/content/abstract/scopus_id/85017518248
Henard, C. A., Guarnieri, M. T., Knoshaug, E. P. (2017). The Chlorella vulgaris S-nitrosoproteome under nitrogen-replete and -deplete conditions. Other. 4(JAN), . https://api.elsevier.com/content/abstract/scopus_id/85038621172
Crawford, M. A., Henard, C. A., Tapscott, T., Porwollik, S., McClelland, M., Vázquez-Torres, A.  (2016). DksA-dependent transcriptional regulation in Salmonella experiencing nitrosative stress. 7(MAR), . https://api.elsevier.com/content/abstract/scopus_id/84964402733
Henard, C. A., Smith, H., Dowe, N., Kalyuzhnaya, M. G., Pienkos, P. T., Guarnieri, M. T. (2016). Bioconversion of methane to lactate by an obligate methanotrophic bacterium. Scientific Reports. 6, . https://api.elsevier.com/content/abstract/scopus_id/84959440704
Carlsen, E. D., Jie, Z., Liang, Y., Henard, C. A., Hay, C., Sun, J., De Matos Guedes, H., Soong, L.  (2015). Interactions between Neutrophils and Leishmania braziliensis Amastigotes Facilitate Cell Activation and Parasite Clearance. Other. 7(4), 354-363. https://api.elsevier.com/content/abstract/scopus_id/84937523457
Henard, C. A., Tapscott, T., Crawford, M. A., Husain, M., Doulias, P. T., Porwollik, S., Liu, L., Mcclelland, M., Ischiropoulos, H., Vázquez-Torres, A.  (2014). The 4-cysteine zinc-finger motif of the RNA polymerase regulator DksA serves as a thiol switch for sensing oxidative and nitrosative stress. Molecular Microbiology. 91(4), 790-804. https://api.elsevier.com/content/abstract/scopus_id/84893808012
Henard, C. A., Carlsen, E. D., Hay, C., Kima, P. E., Soong, L.  (2014). Leishmania amazonensis Amastigotes Highly Express a Tryparedoxin Peroxidase Isoform That Increases Parasite Resistance to Macrophage Antimicrobial Defenses and Fosters Parasite Virulence. PLOS Neglected Tropical Diseases. 8(7), . https://api.elsevier.com/content/abstract/scopus_id/84905483167
Carlsen, E. D., Hay, C., Henard, C. A., Popov, V., Garg, N. J., Soong, L.  (2013). Leishmania amazonensis amastigotes trigger neutrophil activation but resist neutrophil microbicidal mechanisms. Other. 81(11), 3966-3974. https://api.elsevier.com/content/abstract/scopus_id/84886772299
Song, M., Husain, M., Jones-Carson, J., Liu, L., Henard, C. A., Vázquez-Torres, A.  (2013). Low-molecular-weight thiol-dependent antioxidant and antinitrosative defences in Salmonella pathogenesis. Molecular Microbiology. 87(3), 609-622. https://api.elsevier.com/content/abstract/scopus_id/84873060569
Henard, C. A., Vázquez-Torres, A.  (2012). Dksa-dependent resistance of Salmonella enterica Serovar typhimurium against the antimicrobial activity of inducible nitric oxide synthase. Other. 80(4), 1373-1380. https://api.elsevier.com/content/abstract/scopus_id/84860817569
Henard, C. A., Vázquez-Torres, A.  (2011). Nitric oxide and salmonella pathogenesis. Frontiers in Microbiology. 2(APR), . https://api.elsevier.com/content/abstract/scopus_id/84861142087
Henard, C. A., Bourret, T. J., Song, M., Vázquez-Torres, A.  (2010). Control of redox balance by the stringent response regulatory protein promotes antioxidant defenses of Salmonella. Journal of Biological Chemistry. 285(47), 36785-36793. https://api.elsevier.com/content/abstract/scopus_id/78449249372
Phillips, C. D., Henard, C. A., Pfau, R. S. (2007). Amplified fragment length polymorphism and mitochondrial DNA analyses reveal patterns of divergence and hybridization in the hispid cotton rat (Sigmodon hispidus). Journal of Mammalogy. 88(2), 351-359. https://api.elsevier.com/content/abstract/scopus_id/34248370965
Review, Journal
Freed, E., Fenster, J., Smolinski, S. L., Walker, J., Henard, C. A., Gill, R., Eckert, C. A. (2018). Building a genome engineering toolbox in nonmodel prokaryotic microbes. Other. 115(9), 2120-2138. https://api.elsevier.com/content/abstract/scopus_id/85047770407
Henard, C. A., Freed, E. F., Guarnieri, M. T. (2015). Phosphoketolase pathway engineering for carbon-efficient biocatalysis. Current Opinion in Biotechnology. 36, 183-188. https://api.elsevier.com/content/abstract/scopus_id/84941213572
Soong, L., Henard, C. A., Melby, P. C. (2012). Immunopathogenesis of non-healing American cutaneous leishmaniasis and progressive visceral leishmaniasis. Other. 34(6), 735-751. https://api.elsevier.com/content/abstract/scopus_id/84876014017



	
		
		
	  

	

	

  
    
	

	

	  

		

		   
Awarded Grants



  
  
  	  
          
Contracts, Grants and Sponsored Research  

 	 
	 	 
  
Contract   
Henard, C., "Subject Matter Expert-Conversion Verification," Sponsored by National Renewable Energy Laboratory, National, $9024 Funded. (May 1, 2023 – August 31, 2024).
Henard, C., "Subject Matter Expert-Conversion Verification," Sponsored by National Renewable Energy Laboratory, National, $17449 Funded. (August 1, 2019 – May 31, 2023).
Grant - Research   
Henard, C. A. (Principal), "Development of advanced biocatalyst tools and resources to enable biogas-based biomanufacturing.," Sponsored by National Science Foundation, Federal, $716898 Funded. (September 2022 – August 2025).
Henard, C. A., "Development and validation of CRISPR interference genome-wide libraries to enable high-throughput single-carbon biocatalyst optimization.," Sponsored by Department of Energy/MSRDC, Federal, $250000 Funded. (August 2023 – April 2025).
Henard, C. A., Azad, R. K., "Characterization of the carbon dioxide starvation response  in a promising greenhouse gas mitigation biocatalyst.," Sponsored by BioDiscovery Institute, University of North Texas, $30000 Funded. (June 2023 – May 2024).
Henard, C. A., "Identification of CO2- and CbbR-dependent transcriptional responses in methanotroph M. capsulatus," Sponsored by BioDiscovery Institute, University of North Texas, $5000 Funded. (May 2022 – December 2022).
Henard, C. (Principal), "Molecular Coliform Testing," Sponsored by Texas Green Star, LLC, Other, $7347 Funded. (April 1, 2021 – March 2022).
Henard, C., "Combined stable isotope probing and genomics of CH4 and CO2 co-utilization by terrestrial methanotrophic bacteria.," Sponsored by BioDiscovery Institute, University of North Texas, $7500 Funded. (January 1, 2021 – December 31, 2021).
Henard, C., "Support for iGEM research," Sponsored by University of North Texas, University of North Texas, $2500 Funded. (June 1, 2021 – August 1, 2021).
Henard, C., "Elucidation of CO2-dependent methanotrophy at cellular and ecosystem levels.," Sponsored by College of Science, University of North Texas, $10000 Funded. (May 1, 2020 – December 31, 2020).
Grant - Teaching   
Antunes, M. (Principal), Henard, C. A. (Co-Principal), Alberts, A. (Supporting), Hughes, L. E. (Co-Principal), "HHMI SEA-GENES Project," Sponsored by Howard Hughes Medical Institute (HHMI), Private, $11000 Funded. (2019 – Present).
Sponsored Research   
Henard, C. A., "iGEM team registration," Sponsored by UNT Honors College and TAMS, University of North Texas, $5500 Funded. (June 2021 – August 2021).



	
		
		
	  

	

	

	
	

  
  
  

  	

		

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					Overall
Summative Rating		
					Challenge and
Engagement Index		
					Response Rate
					
				

			
			
				

					
					
out of 5		
					
out of 7		
					%
 of 
students responded		
					
					
				

			
		


		
    
			
  • Overall Summative Rating (median):
    
			This rating represents the combined responses of students to the four global summative items and is presented to provide an overall index of the class’s quality. Overall summative statements include the following (response options include a Likert scale ranging from 5 = Excellent, 3 = Good, and 1= Very poor):
				
      
					
    • The course as a whole was
      • 
					
    • The course content was
      • 
					
    • The instructor’s contribution to the course was
      • 
					
    • The instructor’s effectiveness in teaching the subject matter was
      • 
				
    
			
    • 
			
  • Challenge and Engagement Index:
    
			This rating combines student responses to several SPOT items relating to how academically challenging students found the course to be and how engaged they were. Challenge and Engagement Index items include the following (response options include a Likert scale ranging from 7 = Much higher, 4 = Average, and 1 = Much lower):
				
      
					
    • Do you expect your grade in this course to be
      • 
					
    • The intellectual challenge presented was
      • 
					
    • The amount of effort you put into this course was
      • 
					
    • The amount of effort to succeed in this course was
      • 
					
    • Your involvement in course (doing assignments, attending classes, etc.) was
      • 
				
    
			
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