Douglas D. Root

Previous Scheduled Teaching

Published Intellectual Contributions

Abstracts and Proceedings

• Quedan, D., Turner, K.L., Tanner, B.C., Root, D.D. (2023). MYBP-C BINDS MYOSIN S2 IN MOUSE HEART AS REVEALED BY SUPER-RESOLUTION MICROSCOPY. Biophysical Journal. 122 (3s) 1952-Pos. Denton,
• Kampourakis, T., Kalogeris, T., Singh, R., Quedan, D., McDonald, K., Root, D.D. (2023). MYOSIN S2 STABILIZER PEPTIDE DECREASES RECOVERY RATES OF CONTRACTION. Biophysical Journal. 122 (3s) 1952-Pos. Denton, 1155 Union Cir # 305220
• Quedan, D.D., Root, D.D. (2022). ANTI-S2 PEPTIDES AND ANTIBODIES BIND COOPERATIVELY TO THE MYOSIN DIMER WITH HIGH AFFINITY AND STABILIZE THE UNFOLDED STATE OF S2. Biophysical Journal. 140 (3) 22-A-1199-BPS. Denton,
• Root, D.D., Quedan, D.D., Qadan, M.D., Levitan, B.M., Thompson, M.S., Campbell, K. (2022). ANTI-S2 PEPTIDES TARGET CARDIAC MYOSIN FOR IN VIVO RESPONSES. Biophysical Journal. 140 22-A-1075-BPS. Denton,
• Migliore, J.R., Root, D.D. (2022). TRANSMITTED LIGHT IMAGING REVEALS SARCOMERIC ULTRASTRUCTURE IS PRESERVED IN EXPANSION MICROSCOPY. Biophysical Journal. 140 (3) #22-A-1430-BPS. Cell.
• Qadan, M.D., Root, D.D. (2021). The Design of a Destabilizer Peptide to Disrupt SARS-CoV-2 Fusion with Its Targeted Cell Membrane. Biophysical Journal. 120 (3) 204A. Cell.
• Migliore, J.R., Root, D.D. (2021). Using Enzyme-Linked Markers with Chromogenic Substrates in Expansion Microscopy. Biophysical Journal. Biophysical Journal. 120 (3) 360A. Denton, Cell.
• Root, D.D. (2020). BINDING SITE ANALYSIS OF AN ANTI-TROPOMYOSIN DESTABILIZING PEPTIDE USING FLUORESCENCE MICROSCOPY AND SPECTROSCOPY. Biophysical Journal. 108 (3) 276A-277A.
• Aboonsrshiraz, N., Root, D.D. (2020). IMPACT OF ANTI-S2 PEPTIDES ON MYOSIN S2 ISOFORMS AND HCM MUTANTS.. Biophysical Journal. 108 (3) 427A. Cell.
• Tanner, B., Campbell, K., Qadan, M., Aboonsrshiraz, N., Quedan, D., Awinda, P.0., Bernardino-Shafer, A., Guglin, M., Root, D.D. (2019). ANTI-S2 PEPTIDES MODULATE MYOSIN COILED-COIL STRUCTURE AND SHIFT FORCE-pCa CURVES IN HUMAN CARDIAC MUSCLE. Biophysical Journal. Biophysical Journal. 116 (3) 263a. Cell.
• Quedan, D., Bernardino-Schaefer, A., Singh, R., Thang, C., Bhaskaruni, M., Srivastava, R., Root, D.D. (2018). Cooperative Activation of Striated Muscle Thick Filaments by S2 Binding. Texas Journal of Microscopy. 49 (1) 13. Texas, Texas Society of Microscopy. www.texasmicroscopy.org
• Quedan, D., Bernardino-Schaefer, A., singh, R., Thang, C., Bhaskaruni, M., Srivastava, R., Root, D.D. (2018). Cooperative activation of the striated muscle thick filament via S2 binding. Biophysical Journal. 114 (3) 646a. Cambridge, MA, Cell Press. www.cell.com/biophysj/home
• Singh, R., Qadan, M., Aboonsrshiraz, N., Quedan, D., Wang, D., Root, D.D. (2017). Peptide designed to destabilize the myosin coiled coil enhance myofibril shortening while peptides that stabilize the coiled coil inhibit myofibril shortening. Biophysical Journal. 112 557a.
• Singh, R., Zheng, V., Ma, C., Root, D.D. (2016). Competitive Binding to Myosin S2 by C-Protein, Anti-S2 Peptides, and Antibodies Modulate Myosin Coiled-Coil Stability and Myofibril Contractility.. Biophysical Journal. 110 292a.
• Aboonsrshiraz, N., Huang, A., Ma, C., Root, D.D. (2016). Development of High Affinity Anti-S2 Peptides for Stabilizing the Myosin Coiled-Coil. Biophysical Journal. 110 292a.
• Dunn, J., Bih, M., Singh, R., Root, D.D. (2015). Peptide Modulation of Myosin Coiled Coil Stability Monitored with Optical and Force Spectroscopy. Biophysical Journal. 108 (2) 446a.
• Gopal, T., Luchowski, R., Akel, A., Gryczynski, Z., and Borejdo, J.. (2009). "Photoactivatable Quantum Dots in Super-Resolution 3D Microscopy of Myofibrils .".
• Gawalapu, R. K.. (2008). "Computational Estimation of the Energy Requirements for Conformational Transitions in Skeletal Muscle Myosin .".
• Patel, D. and Jin, J.-P.. (2008). "Troponin/Tropomyosin Determines Myosin Target Zones Along the Thin Filament .".
• Gawalapu, R. K.. (2007). "Fluorescent labeling of the myosin strut.".
• Coffee, P.. (2006). "A Novel Fluorescent Nanocircuit Reports Nanometer Scale Conformational Changes Between the Interacting Molecules of Muscle Contraction".
• Coffee, P.. (2006). "A Novel Fluorescent Nanocircuit Reports on the Lever Arm Position of Thin Filament Bound Myosin S1 ".
• Patel, D.. (2006). "LRET-Based 3-D Mapping of Skeletal Troponin T Relative to Myosin S1 Bound to Regulated Actin".
• Hong, F.. (2006). "Measured Variability in the Unwinding of the Myosin Coiled Coil" Related Links:.
• Root, D.D. (2006). "Nanomanipulations and Molecular Simulations of Single Fibrous Muscle Myosin Proteins".
• Root, D.D. (2005). " The structural relationship of muscle myosin to thin filament regulatory proteins".
• Gawalapu, R. K.. (2005). "Computational estimation of the consequences of cleft movement in skeletal muscle myosin.".
• Forbes, J., Yadavalli, V., and Wang, K.. (2005). "Nanomechanics of Coiled-Coil Domains in Myosin".
• Zhang, Z.. (2005). "The Structure of Troponin T N-terminal Tx Motifs upon Ca2+ Binding".
• Patel, D.. (2004). " Resonance Energy Transfer of Pre- and Post-Powerstroke States of Myosin to Troponin.".
• Gundapaneni, D.. (2004). "Does Muscle Crossbridge Flexibility Reside Mostly in the Myosin Head or Subfragment 2?".
• Zhang, Z.. (2004). "Lanthanide Probes for Detecting Insertion, Deletion, or Alternative Splicing Variants.".
• Root, D.D. (2004). "Structural Prediction and Calcium Binding Analysis of a Novel Troponin T N-terminal Fragment.".
• Gundapaneni, D.. (2003). "A Single Molecule Fluorescent Assay Detects Nucleotide-Dependent Effects on Actin and Myosin Crossbridge Flexibility ".
• Zhang, Z. and Jin, J.-P.. (2003). "Binding of Calcium to Avian Pectoral Troponin T".
• Root, D.D. (2002). " Spectroscopic and Computational Analysis of the Actomyosin Interface".
• Root, D.D. (2002). "First Contact of Myosin Binding Actin".
• Xu, J.. (2002). "Myosin Twisting on Actin".
• Xu, J. and Stewart, S.. (2001). "D ocking Atomic Models of Actin and Myosin".
• Xu, J. and Stewart, S.. (2001). "D ocking Atomic Models of Weak and Strong Binding States of Actomyosin by Resonance Energy Transfer".
• Jin, J.-P.. (2001). "Spectroscopic Mapping of Troponin T Reveals Site-Specific Conformational Perturbations by Thin Filament Proteins".
• Xu, J.. (2000). "Conformational States of Myosin Subfragment-1 on Actin,".
• Jin, J.-P.. (2000). "N-terminal Variable Region-Based Modulation of Conformation and Flexibility of Troponin T: A Fluorescence Spectral Study,".
• Hyunguk, P.. (2000). "Noncovalent Crosslinking of SH1 and SH2 in Myosin Demonstrates Dynamic Flexibility of the Melted SH1-SH2 -Helix,".
• Xu, J.. (1999). " Structural Changes at the Actomyosin Interface,".
• Shangguan, X., Xu, J., and McAllister, M. A.. (1999). "Determination of Fluorescent Probe Orientations on Macromolecules by Conformational Searching,".
• Teal, H. E. and Hu, Z.. (1999). "Hydrophobic Modulation Liquid Chromatography for the Purification of Myosin Subfragments.".
• Xu, J.. (1999). "Structural Transitions at the Actomyosin Interface,".
• Shangguan, X., Xu, J., and McAllister, M.. (1998). "Calibrations and Corrections for Probe Size Effects in Resonance Energy Transfer Measurements,".
• Xu, J.. (1998). "Long-Distance Resonance Energy Transfer Between the Regulatory Light Chain and Nucleotide Analogs in Skeletal Muscle Myosin,".
• Miles, J.. (1998). "Sensitized Emission Immuno-Resonance Energy Transfer (SEIRET) Microscopy: Applications to Actin-Associated Proteins,".
• Root, D.D. (1997). "In Situ Molecular Association of Dystrophin with Actin Revealed by Sensitized Emission Immuno-Resonance Energy Transfer,".
• Wang, K.. (1996). "Nebulin Fragment Alters Rigor Binding of Myosin Subfragment-1 to Actin,".
• Wang, K.. (1995). "Nebulin Fragment Binds Both S-1 and Prevents Binding to Actin,".
• Wang, K.. (1995). "Single Nebulin Superrepeat Affects Multiple Actin Protomers in Actomyosin Interaction and Actin Polymerization,".
• Wang, K.. (1994). "Nebulin-Calmodulin As A New Calcium Regulatory System On The Thin Filament Of Skeletal Muscle,".
• Wang, K.. (1993). "Highly Sensitive Silver and Copper-Based Staining Assays for Protein on Blots and Microtiter Plates,".
• Wang, K.. (1993). "Nebulin is a Giant Actin-Binding Protein: High Affinity Binding Sites and Videomicroscopy of Actin-Nebulin,".
• Reisler, E.. (1992). "Catalytic Cooperativity of Modified Myosin Filaments,".
• Reisler, E.. (1991). "Fluorescence Quenching of Etheno-Nucleotides in G- and F-Actin,".
• Reisler, E.. (1990). "Copper Iodide Staining and Determination of Proteins Adsorbed to Microtiter Plates,".
• Snape, W. J., Mayer, E. A., and Williams, R.. (1987). "Disturbance in Colonic Contraction in Colonic Muscle Removed from Patients with Ulcerative-Colitis".

Book

• Berg et al., J.M., Root, D.D. (2015). Clinical Companion to Biochemistry 8th ed.. 2015 (8th) 179 out of 637 pp. New York, NY, W. H. Freeman & Company. https://reg.macmillanhighered.com/Account/Unauthenticated?TargetURL=http%3a%2f%2fwww.macmillanhighered.com%2flaunchpad%2fberg8e%2f1597451

Book Chapter

• Wang, K.. (2002). "Copper Iodide Staining of Proteins," In Basic Protein and Peptide Protocols Second Ed. (Ed. John M. Walker).
• Wang, K.. (2002). "Kinetic Silver Staining of Proteins," In Basic Protein and Peptide Protocols Second Ed. (Ed. John M. Walker).
• Wang, K.. (1996). "Copper Iodide Staining of Proteins on Solid Phases," In Basic Protein and Peptide Protocols (Ed. John M. Walker)..
• Wang, K.. (1996). "Kinetic Silver Staining of Proteins Adsorbed to Microtiter Plates," In Basic Protein and Peptide Protocols (Ed. John M. Walker).

Journal Article

• , D.Q., Singh, R., Akel, A., Bernardino, A.L., Thang, C., Bhaskaruni, M., Haldankar, A., Tanner, B.C., Root, D.D. (2023). Cooperative & competitive binding of anti-myosin tail antibodies revealed by superresolution microscopy. Archives of Biochemistry and Biophysics. Elsevier.
• Singh, R.R., Dunn, J.W., Qadan, M.M., Hall, N., Wang, K.K., Root, D.D. (2018). Data on whole length myosin binding protein C stabilizes myosin S2 as measured by gravitational force spectroscopy. 18 1099-1106. New York, Data in Brief (Elsevier). https://www.sciencedirect.com/science/article/pii/S2352340918303536
• Mondal, H., Louis, J., Archer, L., Patel, M., Nalam, V., Sarowar, S., Sivapalan, V., Root, D.D., Shah, J. (2018). Arabidopsis thaliana ACTIN DEPOLYMERISING FACTOR 3 is required for controlling aphid feeding from the phloem. Plant Physiology. 176 879-890. American Society of Plant Biologists.
• Singh, R.R., Dunn, J.W., Qadan, M.M., Hall, N., Wang, K.K., Root, D.D. (2018). Whole length myosin binding protein C stabilizes myosin S2 as measured by gravitational force spectroscopy. Archives of Biochemistry and Biophysics. 638 41-51. Amsterdam, The Netherlands, Elsevier. https://www.journals.elsevier.com/archives-of-biochemistry-and-biophysics
• Pilar Castro-Zena and Douglas D. Root. (2013). Asymmetric Myosin Binding to the Thin Filament as Revealed by a Fluorescent Nanocircuit. Archives of Biochemistry and Biophysics. 535 (1) 14-21.
• James Dunn and Douglas D. Root. (2011). Demonstrating the uses of the novel Gravitational Force Spectrometer to stretch and measure fibrous proteins.
• Patel, D. A.. (2009). " Close Proximity of Myosin Loop 3 to Troponin Determined by Triangulation of Resonance Energy Transfer Distance Measurements,".
• Yadavalli, V.M., Forbes, J. G., and Wang, K.. (2006). " Coiled-coil Nanomechanics and Uncoiling and Unfolding of the Superhelix and Alpha-Helices of Myosin".
• Hong, F.. (2006). " Downscaling Functional Bioassays by Single Molecule Techniques".
• Gawalapu, R. K.. (2006). " Fluorescence labeling and computational analysis of the strut of myosin's 50 kDa cleft".
• Gundapaneni, D. and Xu, J.. (2005). "High Flexibility of the Actomyosin Crossbridge Resides in Skeletal Muscle Myosin Subfragment-2 as Demonstrated by a New Single Molecule Assay".
• Zhang, Z. and Jin, J.-P.. (2004). " Binding of Calcium Ions to an Avian flight Muscle Troponin T,".
• Vaccaro, C., Zhang, Z., and Castro, M.. (2004). " Detection of Single Nucleotide Variations by a Hybridization Proximity Assay Based on Molecular Beacons and Luminescence Resonance Energy Transfer.".
• Root, D.D. (2002). " A Computational Comparison of the Atomic Models of the Actomyosin Interface".
• Stewart, S., and Xu, J.. (2002). " Dynamic Docking of Myosin and Actin Observed with Resonance Energy Transfer.".
• Root, D.D. (2002). " The Dance of Actin and Myosin: A Review of the Mechanism of Actomyosin Motility.".
• Wang, K.. (2001). "High-Affinity Actin-Binding Nebulin Fragments Influence the ActoS1 Complex,".
• Xu, J.. (2000). "Conformational Selection during Weak Binding at the Actin and Myosin Interface.".
• Jin, J.-P.. (2000). "Modulation of Troponin T Molecular Conformation and Flexibility by Metal Ion Binding to the N-Terminal Variable Region.".
• Teal, H. E. and Hu, Z.. (2000). "Native Purification of Biomolecules with Temperature Mediated Hydrophobic Modulation Liquid Chromatography,".
• Shangguan, X., Xu, J., and McAllister, M.. (1999). " Determination of Fluorescent Probe Orientations on Biomolecules by Conformational Searching: Algorithm Testing and Applications to the Atomic Model of Myosin,".
• Xu, J.. (1998). "Domain Motion between the Regulatory Light Chain and the Nucleotide Site in Skeletal Muscle Myosin,".
• Root, D.D. (1997). "In Situ Molecular Association of Dystrophin with Actin Revealed by Sensitized Emission Immuno-Resonance Energy Transfer,".
• Wang, K.. (1994). "Calmodulin-Sensitive Interaction of Human Nebulin Fragments With Actin And Myosin,".
• Cook, R. K., D., Miller, C., Reisler, E., and Rubinstein, P. A.. (1993). "Enhanced Stimulation of Myosin Subfragment 1 ATPase Activity by Addition of Negatively Charged Residues to the Yeast Actin N-Terminus.".
• Wang, K.. (1993). "Kinetic Silverstaining and Calibration of Proteins Adsorbed to Microtiter Plates.".
• Wang, K.. (1993). "Silver-Enhanced Copper Staining of Protein Blots.".
• Reisler, E.. (1992). "Cooperativity of Thiol-Modified Myosin Filaments: ATPase and Motility Assays of Myosin Function,".
• Reisler, E.. (1992). "The Accessibilities of Etheno-Nucleotides to Collisional Quenchers and the Nucleotide Cleft in G- and F-Actin,".
• Cheung, P., and Reisler, E.. (1991). "Catalytic Cooperativity Induced by SH1 Labeling of Myosin Filaments,".
• Reisler, E.. (1990). "Copper Iodide Staining and Determination of Proteins Adsorbed to Microtiter Plates,".
• Reisler, E.. (1989). "Copper Iodide Staining of Protein Blots on Nitrocellulose Membranes,".
• Snape, W. J., Jr., Mayer, E. A., Koebel, C. B., Hyman, P. E., and Williams, R.. (1989). "Response of Smooth Muscle from Proximal and Distal Human Colon,".
• Snape, W. J., Jr., Hyman, P. E., Mayer, E. A., Sevy, N., and Kao, H. W.. (1987). "Calcium Dependence of Neurotensin Stimulation of Circular Colonic Muscle of the Rabbit,".

Contracts, Grants and Sponsored Research

Grant - Research

• Root, D.D. (Co-Principal), Campbell, K. (Principal), Tanner, B. (Co-Principal), McDonald, K. (Co-Principal), Kampourakis, T. (Co-Principal), "Length-dependent activation in human myocardium," sponsored by NIH, University of North Texas, $2981821 Funded. (2020 - 2024).
• Root, D.D. (Principal), Verbeck, G. (Co-Principal), "Development of Heart-Targeted Therapeutic Peptides and their Characterization by Mass Spectroscopy," sponsored by COS, University of North Texas, $9000 Funded. (2019 - 2019).
• Root, D.D. (Other), "Faculty Research Grants," University of North Texas, $40100 Funded. (1996 - 2014).
• Root, D.D. (Other), "UNT Excellence," sponsored by UNT, University of North Texas, $438343 Funded. (2005 - 2007).
• Neogi, A., Omary, M.A., Root, D.D., Gorman, B., "NSF MRI, Development of Near-field optical microscope," sponsored by National Science Foundation, Federal, $520000 Funded. (2006 - 2007).
• Root, D.D. (Other), "Junior Faculty Summer Research Fellowships," University of North Texas, $10500 Funded. (1996 - 1997).
• Root, D.D. (Principal), "Length-Dependent Activation in Human Myocardium," sponsored by University of Kentucky, NFP, Funded. (2020 - 2024).
• Neogi, A., Omary, M.A., Root, D.D., Gorman, B., "NSF MRI, Development of Near-field optical microscope," sponsored by National Science Foundation, Federal, Funded. (2006 - 2007).