Moo-Yeal D. Lee

Previous Scheduled Teaching

Published Intellectual Contributions

Book Chapter

• Joshi, P., Acharya, P., Zolfaghar, M., Vanga, M., Shrestha, S., Lee, M. (2025). Reproducible, scale-up production of human brain organoids (HBOs) on a pillar plate platform via spheroid transfer. Methods in molecular biology (Clifton, N.J.).
• Shrestha, S., Vanga, M., Jonnadula, C., Acharya, P., Lee, M., Lee, M. (2025). Reproducible, scale-up production of human liver organoids (HLOs) on a pillar plate platform via microarray 3D bioprinting. Methods in molecular biology (Clifton, N.J.).

Journal Article

• Joshi, P., Nascimento, H., Kang, S., Lee, M., Vanga, M., Lee, S., Ku, B., Miranda, M., Lee, M. (2025). Dynamic culture of bioprinted liver tumor spheroids in a pillar/perfusion plate for predictive screening of anticancer drugs. Biotechnology and Bioengineering.
• Lekkala, V., Shrestha, S., Qaryoute, A., Dhinoja, S., Acharya, P., Raheem, A., Jagadeeswaran, P., Lee, M. (2025). Enhanced maturity and functionality of vascular human liver organoids through 3D bioprinting and pillar plate culture. ACS Biomaterials Science & Engineering.
• Lekkala, V., Kang, S., Liu, J., Shrestha, S., Acharya, P., Joshi, P., Jamdagneya, P., Pagnis, S., Kundi, A., Kabbur, S., Zolfaghar, M., Lee, M., Kim, U., Yang, Y., Lee, M. (2024). A pillar/perfusion plate enhances cell growth, reproducibility, throughput, and user friendliness in dynamic 3D cell culture. ACS Biomaterials Science & Engineering.
• Zolfaghar, M., Acharya, P., Joshi, P., Choi, N., Shrestha, S., Lekkala, V., Kang, S., Lee, M., Lee, M. (2024). Cryopreservation of neuroectoderm on a pillar plate and in situ differentiation into human brain organoids. ACS Biomaterials Science & Engineering.
• Acharya, P., Shrestha, S., Joshi, P., Choi, N., Lekkala, V., Kang, S., Lee, M. (2024). Dynamic culture of cerebral organoids using a pillar/perfusion plate for the assessment of developmental neurotoxicity. Biofabrication.
• Joshi, P., Kang, S., Acharya, P., Vanga, M., Lee, M. (2024). High-throughput assessment of metabolism-mediated neurotoxicity by co-culture of neurospheres and liver spheroids. Current Protocols in Toxicology.
• Kim, J., Kim, D., Moon, J., Lee, M., Cho, S. (2024). Oleic acid inhibits the migration and invasion of breast cancer cells with stemness characteristics through oxidative stress-mediated attenuation of the FAK/AKT/NF-κB pathway. Journal of Functional Foods.
• Shrestha, S., Lekkala, V., Acharya, P., Kang, S., Vanga, M., Lee, M. (2024). Reproducible generation of human liver organoids (HLOs) on a pillar plate platform via microarray 3D bioprinting. Lab on a Chip.
• Carney, E., Moslabeh, F., Kang, S., Bunnell, B., Lee, M., Habibi, N. (2024). Self-assembling peptides induced by Eyes absent enzyme to boost the efficacy of doxorubicin therapy in drug-resistant breast cancer cells. Heliyon.
• Acharya, P., Joshi, P., Shrestha, S., Choi, N., Jeong, S., Lee, M. (2024). Uniform cerebral organoid culture on a pillar plate by simple and reproducible spheroid transfer from an ultralow attachment well plate. Biofabrication.
• Lekkala VKR, Kang, S.Y., Liu, J., Shrestha, S., Acharya, P., Joshi, P., Zolfaghar, M., Lee, M.D., Vanga, M.G., Jamdagneya, P., Pagnis, S., Kundi, A., Kabbur, S., Kim, U.T., Yang, Y., Lee, M.Y. (2024). A Pillar/Perfusion Plate Enhances Cell Growth, Reproducibility, Throughput, and User Friendliness in Dynamic 3D Cell Culture.. ACS Biomaterials Science & Engineering. 10 (5) 3478-3488.
• Kang, S., Kimura, M., Shrestha, S., Lewis, P., Lee, S., Cai, Y., Joshi, P., Acharya, P., Liu, J., Yang, Y., Sanchez, J., Ayyagari, S., Alsberg, E., Wells, J., Takebe, T., Lee, M. (2023). A pillar and perfusion plate platform for robust human organoid culture and analysis. Advanced Healthcare Materials.
• Acharya, P., Choi, N., Shrestha, S., Jeong, S., Lee, M. (2023). Brain organoids: A revolutionary tool for modeling neurological disorders and development of therapeutics. Biotechnology and Bioengineering.
• Kim, K., Kim, S., Im, C., Na, K., Lee, M., Park, J., Kuh, H. (2023). Effect of paclitaxel priming on doxorubicin penetration in a multicellular layer model of human colorectal cancer cells. Biochemical and Biophysical Research Communications.
• Joshi, P., Kang, S., Acharya, P., Sidhpura, D., Lee, M. (2023). High-throughput assessment of metabolism-mediated neurotoxicity by combining 3D-cultured neural stem cells and liver cell spheroids. Toxicology in Vitro.
• Kim, H., Kim, H., Nam, M., Park, J., Lee, M., Chung, S., Lee, K., Kuh, H. (2023). Suppression of the antitumoral activity of natural killer cells under indirect coculture with cancer-associated fibroblasts in a pancreatic TIME-on-Chip model. Cancer Cell International.
• Rogozinski, N., Yanez, A., Bhoi, R., Lee, M., Yang, H. (2022). Current methods for fabricating 3D cardiac engineered constructs. iScience.
• Joshi, P., Patel, R., Kang, S., Serbinowski, E., Lee, M. (2022). Establishment of ion channel and ABC transporter assays in 3D-cultrued ReNcell VM on a 384-pillar plate for neurotoxicity potential. Toxicology in Vitro.
• Choi, N., Lee, M., Jeong, S. (2022). Recent advances in 3D-cultured brain tissue models derived from human iPSCs. Biochip Journal.
• Shrestha, S., Lekkala, V., Acharya, P., Siddhpura, D., Lee, M. (2021). Recent advances in microarray 3D bioprinting for high-throughput spheroid and tissue culture and analysis. Essays in Biochemistry.

Contracts, Grants and Sponsored Research

Grant - Research

• Lee, M. (Co-Principal), Joshi, P. (Principal), "Gene-edited liver organoids for predictive hepatotoxicity," sponsored by NIH/NIEHS, Federal, $284638 Funded. (2023 - 2024).
• Lee, M. (Principal), Takebe, T. (Supporting), Wells, J. (Co-Principal), Alsberg, E. (Supporting), "Modeling diabetes using an integrated plate system," sponsored by NIH/NIDDK, Federal, $1278182 Funded. (2021 - 2023).
• Lee, M. (Co-Principal), Joshi, P. (Principal), "Pillar and perfusion well plate platforms for reproducible organoid culture from iPSC," sponsored by NIH/NCATS, Federal, $195946 Funded. (2021 - 2023).
• Lee, M.D. (Principal), Lee, M.D. (Principal), "Pillar and perfusion well plate platforms for reproducible organoid culture from iPSC," sponsored by Bioprinting Laboratories Inc, IND, Funded. (2021 - 2022).
• Lee, M. (Supporting), Lee, M.D. (Principal), "Modeling diabetes using an integrated plate system," sponsored by Children's Hospital Medical Center, NFP, Funded. (2021 - 2021).