Patrick Horn

Previous Scheduled Teaching

Published Intellectual Contributions

Abstracts and Proceedings

• Novotny, L., Dave, P., Horn, P. (2022). Multiple Conserved Cysteine Residues Are Required for Delta‐12 Fatty Acid Desaturase Function in Arabidopsis thaliana. FASEB Journal. 36 The Federation of American Societies for Experimental Biology. https://faseb.onlinelibrary.wiley.com/doi/abs/10.1096/fasebj.2022.36.S1.L7981

Book Chapter

• Horn, P., Chapman, K., Ischebeck, T. (2021). Isolation of Lipid Droplets for Protein and Lipid Analysis. Methods in molecular biology (Clifton, N.J.). Plant Lipids: Methods and Protocols. 2295 295-320. New York, NY, Humana. https://link.springer.com/protocol/10.1007/978-1-0716-1362-7_16

Journal Article

• Cai, Y., Horn, P. (2024). Packaging “vegetable oils”: Insights into plant lipid droplet proteins. Plant Physiology. Oxford University Press (OUP). https://doi.org/10.1093/plphys/kiae533
• Johnston, C., Horn, P., Alonso, A. (2024). First draft reference genome and annotation of the alternative oil species Physaria fendleri. G3: Genes, Genomes, Genetics. https://doi.org/10.1093/g3journal/jkae114
• Horn, P., Cannon, A. (2023). The Molecular Frequency, Conservation and Role of Reactive Cysteines in Plant Lipid Metabolism. Plant and Cell Physiology. Special Issue in Plant and Algal Lipids. 65 (6) 826-844. Oxford University Press. https://doi.org/10.1093/pcp/pcad163
• Horn, P.J., Chapman, K.D. (2023). Imaging plant metabolism in situ. Journal of Experimental Botany. Special Issue in Plant Metabolomics. 75 (6) erad423. Oxford University Press. https://doi.org/10.1093/jxb/erad423
• Borisjuk, L., Horn, P., Chapman, K., Jakob, P., Guendel, A., Rollestschek, H. (2023). Seeing Plants as Never Before. New Phytologist. Tansley review. 238 (5) 1775-1794. https://doi.org/10.1111/nph.18871
• Hoh, D., Horn, P., Kanazawa, A., Froehilch, J., Cruz, J., Tessmer, O.L., Hall, D., Yin, L., Benning, C. (2022). Genetically-determined variations in photosynthesis indicate roles for specific fatty acid species in chilling responses. Plant, Cell & Environment. 45 1682-1697. https://onlinelibrary.wiley.com/doi/10.1111/pce.14313
• Davis Armstrong, N., Spragley, K.J., Chen, W., Hsu, F., Brewer, M., Horn, P., Williams, S., Sale, M., Worrall, B., Keene, K. (2021). Multi-omic analysis of stroke recurrence in African Americans from the Vitamin Intervention for Stroke Prevention (VISP) clinical trial. PLOS One. 16 e0247257. https://doi.org/10.1371/journal.pone.0247257
• Horn, P. (2021). Where Do the Electrons Go? How numerous redox processes drive phytochemical diversity. Phytochemistry Reviews. 20 (2) 367-407. https://doi.org/10.1007/s11101-020-09738-w
• Horn, P., Smith, M., Clark, T., Froehlich, J., Benning, C. (2020). PEROXIREDOXIN Q stimulates the activity of the chloroplast 16:1Δ3trans FATTY ACID DESATURASE4. The Plant journal : for cell and molecular biology. 56 2457-2464. https://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.14657
• Zienkiewicz, A., Zienkiewicz, K., Poliner, E., Pulman, J., Du, Z., Stefano, G., Tsai, C., Horn, P., Feussner, I., Farre, E., Childs, K., Brandizzi, F., Benning, C. (2020). The Microalga Nannochloropsis during transition from quiescence to autotrophy in response to nitrogen availability. Plant Physiology. 182 819-839. http://www.plantphysiol.org/content/early/2019/11/18/pp.19.00854.abstract
• Hurlock, A., Wang, K., Takeuchi, T., Horn, P., Benning, C. (2018). Acyl editing of plastid lipids and chloroplast import of phospahtidylglyerol precursors in Arabidopsis thaliana. The Plant journal : for cell and molecular biology. 95 1128-1139. https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.13999
• Yu, X., Cahoon, R., Horn, P., Shi, H., Prakash, R., Cai, Y., Hearney, M., Chapman, K., Cahoon, E., Schwender, J., Shanklin, J. (2018). Identification of bottlenecks in the accumulation of cyclic fatty acids in the seed oil of Camelina sativa. Plant Biotechnology Journal. 16 (4) 926-938. https://onlinelibrary.wiley.com/doi/full/10.1111/pbi.12839
• Salazar, C., Jones, M., Sturtevant, D., Horn, P., Crossley, J., Zaman, K., Chapman, K., Wrona, M., Isaac, G., Smith, N., Shulaev, V. (2017). Development and application of sub‐2 µm particle CO2‐based chromatography coupled to mass spectrometry for comprehensive analysis of lipids in cottonseed extracts. Rapid communications in mass spectrometry : RCM. 31 (7) 591-605. https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/abs/10.1002/rcm.7825
• Sturtevant, D., Horn, P., Kennedy, C., Hinze, L., Percy, R., Chapman, K. (2017). Lipid metabolites in seeds of diverse Gossypium accessions: molecular identification of a high oleic mutant allele. Planta. 245 595-610. https://link.springer.com/article/10.1007/s00425-016-2630-3
• Campbell, B., Chapman, K., Sturtevant, D., Kennedy, C., Horn, P., Chee, P., Lubbers, E., Meredith Jr., W., Johnson, J., Fraser, D., Jones, D. (2016). Genetic analysis of cottonseed protein and oil in a diverse cotton germplasm. Crop Science. 56 (5) 2457-2464. https://acsess.onlinelibrary.wiley.com/doi/abs/10.2135/cropsci2015.12.0742
• Horn, P., Liu, J., Cocuron, J., McGlew, K., Thrower, N.A., Larson, M., Lu, C., Alonso, A.P., Ohlrogge, J. (2016). Identification of multiple lipid genes with modifications in expression and sequence associated with the evolution of hydroxy fatty acid accumulation in Physaria fendleri. The Plant journal : for cell and molecular biology. 86 (4) 322--348. https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.13163
• Snider, J.L., Collins, G.D., Whitaker, J., Chapman, K.D., Horn, P. (2016). The impact of seed size and chemical composition on seedling vigor, yield, and fiber quality of cotton in five production environments. Field Crops Research. 193 186--195. Elsevier. https://www.sciencedirect.com/science/article/abs/pii/S0378429016301460
• Horn, P., Benning, C. (2016). The plant lipidome in human and environmental health. Science. 353 (6305) 1228-1232. https://www.science.org/doi/abs/10.1126/science.aaf6206
• Hinze, L.L., Horn, P., Kothari, N., Dever, J.K., Frelichowski, J., Chapman, K.D., Percy, R.G. (2015). Nondestructive measurements of cottonseed nutritional trait diversity in the US National Cotton Germplasm Collection. Crop Science. 55 (2) 770--782. The Crop Science Society of America, Inc.. https://acsess.onlinelibrary.wiley.com/doi/abs/10.2135/cropsci2014.04.0318
• Horn, P., Chapman, K.D. (2014). Lipidomics in situ: insights into plant lipid metabolism from high resolution spatial maps of metabolites. Progress in Lipid Research. 54 32--52. Pergamon. https://www.sciencedirect.com/science/article/abs/pii/S0163782714000125
• Vanhercke, T., El Tahchy, A., Liu, Q., Zhou, X., Shrestha, P., Divi, U.K., Ral, J., Mansour, M.P., Nichols, P.D., James, C.N., Horn, P., Chapman, K., Beaudoin, F., Ruiz-Lopez, N., Larkin, P., de Feyter, R., Singh, S., Petrie, J. (2014). Metabolic engineering of biomass for high energy density: oilseed-like triacylglycerol yields from plant leaves. Plant Biotechnology Journal. 12 (2) 231--239. https://onlinelibrary.wiley.com/doi/full/10.1111/pbi.12131
• Horn, P., Chapman, K. (2014). Metabolite Imager: customized spatial analysis of metabolite distributions in mass spectrometry imaging. Metabolomics. 10 337-348. https://link.springer.com/article/10.1007/s11306-013-0575-0
• Horn, P., Sturtevant, D., Chapman, K.D. (2014). Modified oleic cottonseeds show altered content, composition and tissue-specific distribution of triacylglycerol molecular species. Biochimie. 96 28--36. Elsevier. https://www.sciencedirect.com/science/article/abs/pii/S0300908413002800
• Snider, J., Collins, G., Whitaker, J., Chapman, K., Horn, P., Grey, T., others. (2014). Seed size and oil content are key determinants of seedling vigor in Gossypium hirsutum.. Other. 18 (1) 1--9. Cotton Foundation. https://www.cabdirect.org/cabdirect/abstract/20143148867
• Hall, T.D., Chastain, D.R., Horn, P., Chapman, K.D., Choinski, J.S. (2014). Changes during leaf expansion of ΦPSII temperature optima in Gossypium hirsutum are associated with the degree of fatty acid lipid saturation. Journal of Plant Physiology. 171 (6) 411-420. https://api.elsevier.com/content/abstract/scopus_id/84893765909
• Main, C.L., Barber, L.T., Boman, R.K., Chapman, K., Dodds, D.M., Duncan, S., Edmisten, K.L., Horn, P., Jones, M.A., Morgan, G.D., others. (2013). Effects of nitrogen and planting seed size on cotton growth, development, and yield. Agronomy Journal. 105 (6) 1853--1859. The American Society of Agronomy, Inc.. https://acsess.onlinelibrary.wiley.com/doi/abs/10.2134/agronj2013.0154
• Horn, P., James, C.N., Gidda, S.K., Kilaru, A., Dyer, J.M., Mullen, R.T., Ohlrogge, J.B., Chapman, K.D. (2013). Identification of a new class of lipid droplet-associated proteins in plants. Plant Physiology. 162 (4) 1926--1936. American Society of Plant Biologists. https://academic.oup.com/plphys/article/162/4/1926/6110806
• Horn, P., Silva, J.E., Anderson, D., Fuchs, J., Borisjuk, L., Nazarenus, T.J., Shulaev, V., Cahoon, E.B., Chapman, K.D. (2013). Imaging heterogeneity of membrane and storage lipids in transgenic Camelina sativa seeds with altered fatty acid profiles. The Plant journal : for cell and molecular biology. 76 (1) 138--150. https://onlinelibrary.wiley.com/doi/full/10.1111/tpj.12278
• Gidda, S.K., Watt, S.C., Collins-Silva, J., Kilaru, A., Arondel, V., Yurchenko, O., Horn, P., James, C.N., Shintani, D., Ohlrogge, J.B., others. (2013). Lipid droplet-associated proteins (LDAPs) are involved in the compartmentalization of lipophilic compounds in plant cells. Plant Signaling & Behavior. 8 (11) e27141. Taylor \& Francis. https://www.tandfonline.com/doi/full/10.4161/psb.27141
• Park, S., Gidda, S.K., James, C.N., Horn, P., Khuu, N., Seay, D.C., Keereetaweep, J., Chapman, K.D., Mullen, R.T., Dyer, J.M. (2013). The $\alpha$/$\beta$ hydrolase CGI-58 and peroxisomal transport protein PXA1 coregulate lipid homeostasis and signaling in Arabidopsis. The Plant Cell. 25 (5) 1726--1739. American Society of Plant Biologists. https://academic.oup.com/plcell/article/25/5/1726/6097856
• Wang, S., Horn, P., Liou, L.C., Muggeridge, M.I., Zhang, Z., Chapman, K.D., Witt, S.N. (2013). A peroxisome biogenesis deficiency prevents the binding of alpha-synuclein to lipid droplets in lipid-loaded yeast. Biochemical and Biophysical Research Communications. 438 (2) 452-456. https://api.elsevier.com/content/abstract/scopus_id/84882630497
• Horn, P., Chapman, K.D. (2012). Lipidomics in tissues, cells and subcellular compartments. The Plant journal : for cell and molecular biology. 70 (1) 69--80. Blackwell Publishing Ltd Oxford, UK. https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-313X.2011.04868.x
• Horn, P., Joshi, U., Behrendt, A., Chapman, K., Verbeck, G. (2012). On-stage liquid-phase lipid microextraction coupled to nanospray mass spectrometry for detailed, nano-scale lipid analysis. Rapid Communication in Mass Spectrometry. 26 957-962. https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/abs/10.1002/rcm.6194
• Horn, P., Korte, A.R., Neogi, P.B., Love, E., Fuchs, J., Strupat, K., Borisjuk, L., Shulaev, V., Lee, Y., Chapman, K.D. (2012). Spatial mapping of lipids at cellular resolution in embryos of cotton. The Plant Cell. 24 (2) 622--636. American Society of Plant Biologists. https://academic.oup.com/plcell/article/24/2/622/6097124
• Horn, P., Chapman, K. (2011). Organellar lipidomics. Plant Signaling & Behavior. 6 1594-1596. https://www.tandfonline.com/doi/full/10.4161/psb.6.10.17133
• Horn, P., Neogi, P., Tombokan, X., Ghosh, S., Campbell, B.T., Chapman, K.D. (2011). Simultaneous quantification of oil and protein in cottonseed by low-field time-domain nuclear magnetic resonance. Journal of the American Oil Chemists Society (JAOCS). 88 1521--1529. Springer-Verlag. https://link.springer.com/article/10.1007/s11746-011-1829-5
• Adeyo, O., Horn, P., Lee, S., Binns, D.D., Chandrahas, A., Chapman, K.D., Goodman, J.M. (2011). The yeast lipin orthologue Pah1p is important for biogenesis of lipid droplets. Journal of Cell Biology. 192 (6) 1043--1055. The Rockefeller University Press. https://rupress.org/jcb/article/192/6/1043/36233/The-yeast-lipin-orthologue-Pah1p-is-important-for
• Horn, P., Ledbetter, N.R., James, C.N., Hoffman, W.D., Case, C.R., Verbeck, G.F., Chapman, K.D. (2011). Visualization of lipid droplet composition by direct organelle mass spectrometry. Journal of Biological Chemistry. 286 (5) 3298--3306. Elsevier. https://www.jbc.org/article/S0021-9258(20)54035-0/fulltext
• James, C.N., Horn, P., Case, C.R., Gidda, S.K., Zhang, D., Mullen, R.T., Dyer, J.M., Anderson, R.G., Chapman, K.D. (2010). Disruption of the Arabidopsis CGI-58 homologue produces Chanarin--Dorfman-like lipid droplet accumulation in plants. Proceedings of the National Academy of Sciences. 107 (41) 17833--17838. National Academy of Sciences. https://www.pnas.org/doi/abs/10.1073/pnas.0911359107

Contracts, Grants and Sponsored Research

Grant - Research

• Ayre, B.G., McGarry, R.C., Macias, V.M., Horn, P., Shah, J., "A Laser Microdissection System to Enhance Agricultural and Food Research in the North Texas and Southern Oklahoma Region," sponsored by USDA NIFA Equipment Grants Program, Federal, $341019 Funded. (2023 - 2027).
• Horn, P., Cai, Y., "Next-Generation Plant Lipid Droplets: Tailoring Organelle Function for Diverse Bioproducts," sponsored by BioDiscovery Institute, University of North Texas, $20000 Funded. (2025 - 2026).
• Horn, P. (Principal), Chapman, K. (Co-Principal), "Elucidating Cyclic Fatty Acid Biosynthesis and Compartmentalization to Improve Cottonseed Value," sponsored by USDA-NIFA, Federal, $294000 Funded. (2022 - 2025).